VCU 10x Genomics Shared Resource

10x Genomics Chromium Controller

About this resource

The State Council of Higher Education for Virginia and Virginia College Building Authority (VCBA) jointly administered Higher Education Equipment Trust Fund (HEETF) graciously supported the acquisition of the 10x Genomics Chromium Controller at VCU. The instrument is housed in the laboratory of Chuck Harrell, PhD, within the Department of Pathology, located on the 4th floor of Sanger Hall.

The 10x Genomics Chromium Controller and associated application kits enable immunology researchers to go beyond traditional cellular phenotyping approaches and obtain a more complete view of innate and adaptive immunity.  Applications are highlighted below.

Single Cell Immune Profiling

The Chromium Single Cell Immune Profiling Solution is a comprehensive, scalable tool for profiling full-length paired V(D)J transcripts from hundreds to millions of lymphocytes. The new solution enables assembly of full-length V(D)J sequences on a cell-by-cell basis, providing high resolution insights into the adaptive immune system.

The Chromium Single Cell Immune Profiling Solution allows you to simultaneously examine the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells in human or mouse on a cell-by-cell basis.

Single Cell Immune Sequencing

Feature Barcoding for Cell Surface Protein Expression and Antigen Specificity Determination

Single Cell Gene Expression

The Chromium Single Cell Gene Expression Solution provides high-throughput, single cell expression measurements that enable discovery of gene expression dynamics and molecular profiling of individual cell types.

The Chromium Single Cell Gene Expression Solution provides an unparalleled approach to uncover cell-to-cell gene expression variability and identify rare cell types from complex biological samples.

Single Cell ATAC

The Chromium Single Cell ATAC Solution provides genome-wide chromatin accessibility information for tens of thousands of nuclei. This allows discovery of cellular heterogeneity stemming from epigenetic variability and a better understanding of the regulation of genes, to better define cell state and types for lineage tracing and biomarker discovery at high-resolution.

Single Cell CNV

The Chromium Single Cell Copy Number Variation (CNV) Solution provides a comprehensive, scalable solution for revealing genome heterogeneity and understanding clonal evolution. Enables the study of disease pathogenesis and mosaicism at the single cell level.

Exome Sequencing

The Chromium Exome Solution uses the power of linked-reads to fully resolve genic phasing, structural variation, and detect variants in previously inaccessible and complex regions of the exome.

Genome Sequencing

The Chromium Genome Solution provides long range information on a genome-wide scale, including variant calling, phasing and extensive characterization of genomic structure. Useful for characterizing complex variants in heritable disorders, and discovering key genomic alterations in cancer.